In Vivo Imaging of T Cell Immunological Synapses and Kinapses in Lymph Nodes.
Methods Mol Biol
; 1584: 559-568, 2017.
Article
em En
| MEDLINE
| ID: mdl-28255726
ABSTRACT
T cells can become activated in lymph nodes following a diverse set of interactions with antigen-presenting cells. These cellular contacts range from short and dynamic to stable and long-lasting interactions, termed kinapses and synapses, respectively. Here, we describe a methodology to generate naïve T cells expressing a fluorescent probe of interest through the generation of bone marrow chimeras and to image T cell dynamics using intravital two-photon microscopy. In these settings, the formation of kinapses and synapses can be triggered by the administration of low and high affinity peptides, respectively. Finally, 3D cell tracking can help classify distinct T cell behaviors. These approaches should offer new possibilities for dissecting the process of T cell activation in vivo.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos T
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Microscopia de Fluorescência por Excitação Multifotônica
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Sinapses Imunológicas
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Microscopia Intravital
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Linfonodos
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article