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RXLR Effector AVR2 Up-Regulates a Brassinosteroid-Responsive bHLH Transcription Factor to Suppress Immunity.
Turnbull, Dionne; Yang, Lina; Naqvi, Shaista; Breen, Susan; Welsh, Lydia; Stephens, Jennifer; Morris, Jenny; Boevink, Petra C; Hedley, Pete E; Zhan, Jiasui; Birch, Paul R J; Gilroy, Eleanor M.
Afiliação
  • Turnbull D; Cell and Molecular Science, James Hutton Institute (D.T., L.Y., S.N., L.W., J.S., J.M., P.C.B., P.E.H., P.R.J.B., E.M.G.), and Division of Plant Science, School of Life Science (at The James Hutton institute) (D.T., L.Y., S.N., P.R.J.B.), University of Dundee Invergowrie, Dundee, DD2 5DA, United Kin
  • Yang L; Fujian Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China (L.Y., J.Z.).
  • Naqvi S; James Hutton Ltd (at The James Hutton Institute), Plant Sciences Division, Research School of Biology, The Australian National University, Canberra 2601, Australia (S.B.); and.
  • Breen S; Effector Consortium, James Hutton Institute, Invergowrie, Dundee DD2 5DA, United Kingdom (D.T., L.Y., S.N., L.W., P.C.B., P.E.H., P.R.J.B., E.M.G.).
  • Welsh L; Cell and Molecular Science, James Hutton Institute (D.T., L.Y., S.N., L.W., J.S., J.M., P.C.B., P.E.H., P.R.J.B., E.M.G.), and Division of Plant Science, School of Life Science (at The James Hutton institute) (D.T., L.Y., S.N., P.R.J.B.), University of Dundee Invergowrie, Dundee, DD2 5DA, United Kin
  • Stephens J; Fujian Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China (L.Y., J.Z.).
  • Morris J; James Hutton Ltd (at The James Hutton Institute), Plant Sciences Division, Research School of Biology, The Australian National University, Canberra 2601, Australia (S.B.); and.
  • Boevink PC; Effector Consortium, James Hutton Institute, Invergowrie, Dundee DD2 5DA, United Kingdom (D.T., L.Y., S.N., L.W., P.C.B., P.E.H., P.R.J.B., E.M.G.).
  • Hedley PE; Cell and Molecular Science, James Hutton Institute (D.T., L.Y., S.N., L.W., J.S., J.M., P.C.B., P.E.H., P.R.J.B., E.M.G.), and Division of Plant Science, School of Life Science (at The James Hutton institute) (D.T., L.Y., S.N., P.R.J.B.), University of Dundee Invergowrie, Dundee, DD2 5DA, United Kin
  • Zhan J; Fujian Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China (L.Y., J.Z.).
  • Birch PRJ; James Hutton Ltd (at The James Hutton Institute), Plant Sciences Division, Research School of Biology, The Australian National University, Canberra 2601, Australia (S.B.); and.
  • Gilroy EM; Effector Consortium, James Hutton Institute, Invergowrie, Dundee DD2 5DA, United Kingdom (D.T., L.Y., S.N., L.W., P.C.B., P.E.H., P.R.J.B., E.M.G.).
Plant Physiol ; 174(1): 356-369, 2017 May.
Article em En | MEDLINE | ID: mdl-28270626
ABSTRACT
An emerging area in plant research focuses on antagonism between regulatory systems governing growth and immunity. Such cross talk represents a point of vulnerability for pathogens to exploit. AVR2, an RXLR effector secreted by the potato blight pathogen Phytophthora infestans, interacts with potato BSL1, a putative phosphatase implicated in growth-promoting brassinosteroid (BR) hormone signaling. Transgenic potato (Solanum tuberosum) plants expressing the effector exhibit transcriptional and phenotypic hallmarks of overactive BR signaling and show enhanced susceptibility to P. infestans Microarray analysis was used to identify a set of BR-responsive marker genes in potato, all of which are constitutively expressed to BR-induced levels in AVR2 transgenic lines. One of these genes was a bHLH transcription factor, designated StCHL1, homologous to AtCIB1 and AtHBI1, which are known to facilitate antagonism between BR and immune responses. Transient expression of either AVR2 or CHL1 enhanced leaf colonization by P. infestans and compromised immune cell death activated by perception of the elicitin Infestin1 (INF1). Knockdown of CHL1 transcript using Virus-Induced Gene Silencing (VIGS) reduced colonization of P. infestans on Nicotiana benthamiana Moreover, the ability of AVR2 to suppress INF1-triggered cell death was attenuated in NbCHL1-silenced plants, indicating that NbCHL1 was important for this effector activity. Thus, AVR2 exploits cross talk between BR signaling and innate immunity in Solanum species, representing a novel, indirect mode of innate immune suppression by a filamentous pathogen effector.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Solanum tuberosum / Fatores de Virulência / Fatores de Transcrição Hélice-Alça-Hélice Básicos / Phytophthora infestans Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Solanum tuberosum / Fatores de Virulência / Fatores de Transcrição Hélice-Alça-Hélice Básicos / Phytophthora infestans Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2017 Tipo de documento: Article