Establishment of stable mouse myeloma cells constitutively secreting human tissue-type plasminogen activator.
Gene
; 57(1): 131-41, 1987.
Article
em En
| MEDLINE
| ID: mdl-2828179
ABSTRACT
Expression plasmids for human tissue-type plasminogen activator (t-pA) were introduced into mouse myeloma cells and stable cell lines constitutively secreting t-pA established by selection with mycophenolic acid. Expression of t-pA is driven either by the simian virus 40 early promoter or by immunoglobulin regulatory elements of either light or heavy chains of the mouse. The availability of myeloma cells secreting a heterologous protein is of importance for biotechnological applications, because large-scale fermentation of myeloma cells is well established.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ativador de Plasminogênio Tecidual
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article