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Global reorganisation of cis-regulatory units upon lineage commitment of human embryonic stem cells.
Freire-Pritchett, Paula; Schoenfelder, Stefan; Várnai, Csilla; Wingett, Steven W; Cairns, Jonathan; Collier, Amanda J; García-Vílchez, Raquel; Furlan-Magaril, Mayra; Osborne, Cameron S; Fraser, Peter; Rugg-Gunn, Peter J; Spivakov, Mikhail.
Afiliação
  • Freire-Pritchett P; Nuclear Dynamics Programme, Babraham Institute, Cambridge, United Kingdom.
  • Schoenfelder S; Nuclear Dynamics Programme, Babraham Institute, Cambridge, United Kingdom.
  • Várnai C; Nuclear Dynamics Programme, Babraham Institute, Cambridge, United Kingdom.
  • Wingett SW; Nuclear Dynamics Programme, Babraham Institute, Cambridge, United Kingdom.
  • Cairns J; Nuclear Dynamics Programme, Babraham Institute, Cambridge, United Kingdom.
  • Collier AJ; Epigenetics Programme, Babraham Institute, Cambridge, United Kingdom.
  • García-Vílchez R; Wellcome Trust - Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom.
  • Furlan-Magaril M; Epigenetics Programme, Babraham Institute, Cambridge, United Kingdom.
  • Osborne CS; Nuclear Dynamics Programme, Babraham Institute, Cambridge, United Kingdom.
  • Fraser P; Department of Genetics and Molecular Medicine, King's College London School of Medicine, London, United Kingdom.
  • Rugg-Gunn PJ; Nuclear Dynamics Programme, Babraham Institute, Cambridge, United Kingdom.
  • Spivakov M; Epigenetics Programme, Babraham Institute, Cambridge, United Kingdom.
Elife ; 62017 03 23.
Article em En | MEDLINE | ID: mdl-28332981
Long-range cis-regulatory elements such as enhancers coordinate cell-specific transcriptional programmes by engaging in DNA looping interactions with target promoters. Deciphering the interplay between the promoter connectivity and activity of cis-regulatory elements during lineage commitment is crucial for understanding developmental transcriptional control. Here, we use Promoter Capture Hi-C to generate a high-resolution atlas of chromosomal interactions involving ~22,000 gene promoters in human pluripotent and lineage-committed cells, identifying putative target genes for known and predicted enhancer elements. We reveal extensive dynamics of cis-regulatory contacts upon lineage commitment, including the acquisition and loss of promoter interactions. This spatial rewiring occurs preferentially with predicted changes in the activity of cis-regulatory elements and is associated with changes in target gene expression. Our results provide a global and integrated view of promoter interactome dynamics during lineage commitment of human pluripotent cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Regulação da Expressão Gênica / Células-Tronco Embrionárias Humanas Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Regulação da Expressão Gênica / Células-Tronco Embrionárias Humanas Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article