The RAB GTPase RAB18 modulates macroautophagy and proteostasis.
Biochem Biophys Res Commun
; 486(3): 738-743, 2017 05 06.
Article
em En
| MEDLINE
| ID: mdl-28342870
ABSTRACT
Macroautophagy is a conserved degradative pathway and its deterioration is linked to disturbances in cellular proteostasis and multiple diseases. Here, we show that the RAB GTPase RAB18 modulates autophagy in primary human fibroblasts. The knockdown of RAB18 results in a decreased autophagic activity, while its overexpression enhances the degradative pathway. Importantly, this function of RAB18 is dependent on RAB3GAP1 and RAB3GAP2, which might act as RAB GEFs and stimulate the activity of the RAB GTPase. Moreover, the knockdown of RAB18 deteriorates proteostasis and results in the intracellular accumulation of ubiquitinated degradation-prone proteins. Thus, the RAB GTPase RAB18 is a positive modulator of autophagy and is relevant for the maintenance of cellular proteostasis.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Autofagia
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Proteínas rab de Ligação ao GTP
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Proteínas rab3 de Ligação ao GTP
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Fibroblastos
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article