Sensitivity of tumor surface brushings to detect human papilloma virus DNA in head and neck cancer.
Oral Oncol
; 67: 103-108, 2017 04.
Article
em En
| MEDLINE
| ID: mdl-28351563
ABSTRACT
OBJECTIVE:
Human papilloma virus (HPV) induced head and neck squamous cell carcinoma (HNSCC) represents a distinct tumor subset. We questioned how accurately a brushing from the tumor surface detects HPV in patients with HNSCC. MATERIALS ANDMETHODS:
Brushings from the tumor surface were compared with HPV DNA isolation from formalin-fixed and paraffin-embedded (FFPE) tumor biopsies, which served as the reference standard. In both matrices, HPV DNA was detected using a commercially available test kit. In addition, p16 was assessed in tumor biopsies by immunohistochemistry (IHC). The tumors were considered p16 positive if 70% or more of cancer cells expressed p16.RESULTS:
93 patients with HNSCC were included. Sensitivity and specificity of the brush test were 83% (95%CI 67-92%) and 85% (95%CI 72-93%). Results of p16 IHC were concordant with FFPE samples DNA determinations in 73/93 patients. In 53 patients (57%) the tumor was located in the oropharynx and in 40 patients (43%) the tumor was located in the non-oropharynx region. Sensitivity and specificity of the brush test in patients with oropharyngeal cancer was higher with 86% (95%CI 70-95%) and 89% (95%CI 65-99%).CONCLUSION:
Superficial brushes from the tumor surface may be used to identify HPV positive HNSCC.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Papillomaviridae
/
DNA Viral
/
Neoplasias de Cabeça e Pescoço
Tipo de estudo:
Diagnostic_studies
Limite:
Aged
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Aged80
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Female
/
Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article