MK-801-Treated Oligodendrocytes as a Cellular Model to Study Schizophrenia.
Adv Exp Med Biol
; 974: 269-277, 2017.
Article
em En
| MEDLINE
| ID: mdl-28353246
ABSTRACT
Glutamate is the most important excitatory neurotransmitter in the brain. The N-methyl-D-aspartate (NMDA) subtype of glutamate receptor is found both in neurons and glial cells such as oligodendrocytes, which have been shown to be dysfunctional in schizophrenia. For this reasons, the oligodendrocyte MO3.13 cell line has been used to study glutamatergic dysfunction as a model of schizophrenia using the NMDA receptor antagonists such as MK-801 to block receptor function. Here, we describe a comprehensive protocol for culturing and carrying out proteomic analyses of MK-801-treated MO3.13 cells as a means of identifying potential new biomarkers and targets for drug discovery in schizophrenia research.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esquizofrenia
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Oligodendroglia
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Maleato de Dizocilpina
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Antagonistas de Aminoácidos Excitatórios
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article