Targeted Diazotransfer Reagents Enable Selective Modification of Proteins with Azides.
Bioconjug Chem
; 28(4): 913-917, 2017 04 19.
Article
em En
| MEDLINE
| ID: mdl-28355874
ABSTRACT
In chemical biology, azides are used to chemically manipulate target structures in a bioorthogonal manner for a plethora of applications ranging from target identification to the synthesis of homogeneously modified protein conjugates. While a variety of methods have been established to introduce the azido group into recombinant proteins, a method that directly converts specific amino groups in endogenous proteins is lacking. Here, we report the first biotin-tethered diazotransfer reagent DtBio and demonstrate that it selectively modifies the model proteins streptavidin and avidin and the membrane protein BioY on cell surface. The reagent converts amines in the proximity of the binding pocket to azides and leaves the remaining amino groups in streptavidin untouched. Reagents of this novel class will find use in target identification as well as the selective functionalization and bioorthogonal protection of proteins.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Azidas
/
Proteínas de Bactérias
/
Biotina
/
Avidina
/
Lactococcus lactis
/
Estreptavidina
/
Escherichia coli
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article