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High-Throughput and Reliable Isotope Label-free Approach for Profiling 24 Metabolic Enzymes in FVB Mice and Sex Differences.
Chen, Jiamei; Zhu, Lijun; Li, Xiaoyan; Zheng, Haihui; Yan, Tongmeng; Xie, Cong; Zeng, Sijing; Yu, Jia; Jiang, Huangyu; Lu, Linlin; Qi, Xiaoxiao; Wang, Ying; Hu, Ming; Liu, Zhongqiu.
Afiliação
  • Chen J; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China (J.C., L.Z., X.L., H.Z., S.Z., J.Y., H.J., L.L., X.Q., Y.W., M.H., Z.L.); State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Te
  • Zhu L; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China (J.C., L.Z., X.L., H.Z., S.Z., J.Y., H.J., L.L., X.Q., Y.W., M.H., Z.L.); State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Te
  • Li X; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China (J.C., L.Z., X.L., H.Z., S.Z., J.Y., H.J., L.L., X.Q., Y.W., M.H., Z.L.); State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Te
  • Zheng H; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China (J.C., L.Z., X.L., H.Z., S.Z., J.Y., H.J., L.L., X.Q., Y.W., M.H., Z.L.); State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Te
  • Yan T; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China (J.C., L.Z., X.L., H.Z., S.Z., J.Y., H.J., L.L., X.Q., Y.W., M.H., Z.L.); State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Te
  • Xie C; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China (J.C., L.Z., X.L., H.Z., S.Z., J.Y., H.J., L.L., X.Q., Y.W., M.H., Z.L.); State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Te
  • Zeng S; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China (J.C., L.Z., X.L., H.Z., S.Z., J.Y., H.J., L.L., X.Q., Y.W., M.H., Z.L.); State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Te
  • Yu J; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China (J.C., L.Z., X.L., H.Z., S.Z., J.Y., H.J., L.L., X.Q., Y.W., M.H., Z.L.); State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Te
  • Jiang H; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China (J.C., L.Z., X.L., H.Z., S.Z., J.Y., H.J., L.L., X.Q., Y.W., M.H., Z.L.); State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Te
  • Lu L; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China (J.C., L.Z., X.L., H.Z., S.Z., J.Y., H.J., L.L., X.Q., Y.W., M.H., Z.L.); State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Te
  • Qi X; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China (J.C., L.Z., X.L., H.Z., S.Z., J.Y., H.J., L.L., X.Q., Y.W., M.H., Z.L.); State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Te
  • Wang Y; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China (J.C., L.Z., X.L., H.Z., S.Z., J.Y., H.J., L.L., X.Q., Y.W., M.H., Z.L.); State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Te
  • Hu M; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China (J.C., L.Z., X.L., H.Z., S.Z., J.Y., H.J., L.L., X.Q., Y.W., M.H., Z.L.); State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Te
  • Liu Z; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China (J.C., L.Z., X.L., H.Z., S.Z., J.Y., H.J., L.L., X.Q., Y.W., M.H., Z.L.); State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Te
Drug Metab Dispos ; 45(6): 624-634, 2017 06.
Article em En | MEDLINE | ID: mdl-28356314
FVB mice are extensively used in transgenic and pharmacokinetic research. In this study, a validated isotope label-free method was constructed using ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to quantify 24 drug-metabolizing enzymes (DMEs) in FVB mice. The DMEs include cytochrome P450s (CYP450s/Cyp450s), UDP-glucuronsyltransferases (UGTs/Ugts), and sulfotransferases (SULTs/Sults), which catalyze a variety of reactions to detoxify xenobiotics and endobiotics. The proposed UHPLC-MS/MS method exhibited good range and high sensitivity for signature peptides, as well as acceptable accuracy, precision, and recovery. The protein expression profiles of the DMEs were determined in male and female mice. Overall, the major Cyps, Ugts, and Sults were expressed in male mice followed the rank order: Cyp2c29 > 2e1 > 3a11 > 1a2 > 2d22 > 27a1 > 2c39; Ugt2b5 > 2b1 > 1a6a > 1a9 > 1a1 > 2a3 > 1a2 > 1a5; and Sult1a1 > 3a > 1d1. In contrast, the rank order in female mice was Cyp2c29 > 2e1 > 2c39 > 2d22 > 3a11 > 1a2 > 27a1; Ugt1a6a > 2b5 > 1a1 > 2b1 > 2a3 > 1a9 > 1a5 > 1a2; and Sult1a1 > 3a1 > 1d1. Cyp2c29, Cyp1a2, Cyp27a1, Ugt2b1, Ugt2b5 and Ugt2b36 were male predominant, whereas Cyp2c39, Cyp2d22, Cyp7a1, Ugt1a1, Ugt1a5, Sult1a1, Sult3a1, and Sult1d1 were female predominant. This work could serve as a useful reference for the metabolic study of new drugs and for elucidating the effectiveness and toxicity of drugs. The method is stable, simple, and rapid for determining the expression of DMEs in animals.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Enzimas / Metaboloma / Isótopos Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Enzimas / Metaboloma / Isótopos Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article