Improved campesterol production in engineered Yarrowia lipolytica strains.
Biotechnol Lett
; 39(7): 1033-1039, 2017 Jul.
Article
em En
| MEDLINE
| ID: mdl-28357621
ABSTRACT
OBJECTIVES:
To engineer Yarrowia lipolytica for improving the heterologous production of campesterol (a key precursor to manufacture pharmaceutical steroids).RESULTS:
By screening 7-dehydrocholesterol reductase (DHCR7) from diverse species, DHCR7 from Danio rerio was the best candidate for campesterol synthesis. Overexpression of ACL (ATP citrate lyase) or POX2 (peroxisome acyl-CoA oxidase 2) were key to improving campesterol production. The highest yield of campesterol was 942 mg/l was with the strain overexpressing POX2 in a 5 l bioreactor via high cell density fermentation process with a restricted supply of carbon sourc, sunflower seed oil.CONCLUSIONS:
A promising platform to synthesize downstream steroid drugs was established. Efficient approaches were provided to improve the production of desired molecules in Y. lipolytica with high oil utilization efficiency.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fitosteróis
/
Colesterol
/
Yarrowia
/
Vias Biossintéticas
/
Engenharia Metabólica
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article