Evidence of simian retrovirus type D by polymerase chain reaction.
J Med Primatol
; 46(3): 79-86, 2017 06.
Article
em En
| MEDLINE
| ID: mdl-28370081
ABSTRACT
BACKGROUND:
Over the past few years, there have been reports of finding Simian retrovirus type D (SRV) in macaque colonies where some animals were characterized as antibody positive but virus negative raising questions about how SRV was transmitted or whether there is a variant strain detected by antibody but not polymerase chain reaction (PCR) in current use.METHODS:
We developed a three-round nested PCR assay using degenerate primers targeting the pol gene to detect for SRV serotypes 1-5 and applied this newly validated PCR assay to test macaque DNA samples collected in China from 2010 to 2015.RESULTS:
Using the nested PCR assay validated in this study, we found 0.15% of the samples archived on FTA® cards were positive.CONCLUSIONS:
The source of SRV infection identified within domestic colonies might have originated from imported macaques. The multiplex nested PCR assay developed here may supplement the current assays for SRV.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções Tumorais por Vírus
/
Reação em Cadeia da Polimerase
/
Infecções por Retroviridae
/
Retrovirus dos Símios
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Macaca fascicularis
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Macaca mulatta
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Doenças dos Macacos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article