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Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB.
Fazli, Mustafa; Rybtke, Morten; Steiner, Elisabeth; Weidel, Elisabeth; Berthelsen, Jens; Groizeleau, Julie; Bin, Wu; Zhi, Boo Zhao; Yaming, Zhang; Kaever, Volkhard; Givskov, Michael; Hartmann, Rolf W; Eberl, Leo; Tolker-Nielsen, Tim.
Afiliação
  • Fazli M; Department of Biology, Faculty of Science, University of Copenhagen, Copenhagen, Denmark.
  • Rybtke M; Costerton Biofilm Center, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Steiner E; Costerton Biofilm Center, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Weidel E; Department of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland.
  • Berthelsen J; Department of Drug Design and Optimization, Helmholtz Institute for Pharmaceutical Research Saarland, Saarbrücken, Germany.
  • Groizeleau J; Costerton Biofilm Center, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Bin W; Costerton Biofilm Center, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Zhi BZ; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
  • Yaming Z; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
  • Kaever V; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
  • Givskov M; Research Core Unit Metabolomics, Institute of Pharmacology, Hannover Medical School, Hannover, Germany.
  • Hartmann RW; Costerton Biofilm Center, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Eberl L; Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore.
  • Tolker-Nielsen T; Department of Drug Design and Optimization, Helmholtz Institute for Pharmaceutical Research Saarland, Saarbrücken, Germany.
Microbiologyopen ; 6(4)2017 08.
Article em En | MEDLINE | ID: mdl-28419759
Knowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm-control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c-di-GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c-di-GMP effectors is needed. We provide evidence that c-di-GMP, the alternative sigma factor RpoN (σ54), and the enhancer-binding protein BerB play a role in biofilm formation of Burkholderia cenocepacia by regulating the production of a biofilm-stabilizing exopolysaccharide. Our findings suggest that BerB binds c-di-GMP, and activates RpoN-dependent transcription of the berA gene coding for a c-di-GMP-responsive transcriptional regulator. An increased level of the BerA protein in turn induces the production of biofilm-stabilizing exopolysaccharide in response to high c-di-GMP levels. Our findings imply that the production of biofilm exopolysaccharide in B. cenocepacia is regulated through a cascade involving two consecutive transcription events that are both activated by c-di-GMP. This type of regulation may allow tight control of the expenditure of cellular resources.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fator sigma / Fatores de Transcrição / GMP Cíclico / Biofilmes / Burkholderia cenocepacia Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fator sigma / Fatores de Transcrição / GMP Cíclico / Biofilmes / Burkholderia cenocepacia Idioma: En Ano de publicação: 2017 Tipo de documento: Article