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Complete nucleotide sequence and annotation of the temperate corynephage ϕ16 genome.
Lobanova, Juliya S; Gak, Evgueni R; Andreeva, Irina G; Rybak, Konstantin V; Krylov, Alexander A; Mashko, Sergey V.
Afiliação
  • Lobanova JS; Ajinomoto-Genetika Research Institute, 1st Dorozhny pr. 1-1, Moscow, 117545, Russian Federation. Yulia_Lobanova@agri.ru.
  • Gak ER; Ajinomoto-Genetika Research Institute, 1st Dorozhny pr. 1-1, Moscow, 117545, Russian Federation.
  • Andreeva IG; Ajinomoto-Genetika Research Institute, 1st Dorozhny pr. 1-1, Moscow, 117545, Russian Federation.
  • Rybak KV; Ajinomoto-Genetika Research Institute, 1st Dorozhny pr. 1-1, Moscow, 117545, Russian Federation.
  • Krylov AA; Ajinomoto-Genetika Research Institute, 1st Dorozhny pr. 1-1, Moscow, 117545, Russian Federation.
  • Mashko SV; Ajinomoto-Genetika Research Institute, 1st Dorozhny pr. 1-1, Moscow, 117545, Russian Federation.
Arch Virol ; 162(8): 2489-2492, 2017 Aug.
Article em En | MEDLINE | ID: mdl-28455670
ABSTRACT
The complete genome of ϕ16, a temperate corynephage from Corynebacterium glutamicum ATCC 21792, was sequenced and annotated (GenBank KY250482). The electron microscopy study of ϕ16 virion confirmed that it belongs to the family Siphoviridae. The ϕ16 genome consists of a linear double-stranded DNA molecule of 58,200 bp (G+C = 52.2%) with protruding cohesive 3'-ends of 14 nt. Four major structural proteins were separated by SDS-PAGE and identified by peptide mass fingerprinting technique. Using bioinformatics analysis, 101 putative ORFs and 5 tRNA genes were predicted. Only 27 putative gene products could be assigned to known biological functions. The ϕ16 genome was divided into functional modules. Seven putative promoters and eight putative unidirectional intrinsic terminators were predicted. One site of putative «-1¼ programmed ribosomal frameshifting was proposed in the phage tail assembly genome region. C. glutamicum genetic tools could be broadened by exploiting the known integrase gene (gp33) and the newly identified excisionase gene (gp47), participating in site-specific recombination between ϕ16-attP/attB.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Viral / Genoma Viral / Siphoviridae / Corynebacterium glutamicum Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Viral / Genoma Viral / Siphoviridae / Corynebacterium glutamicum Idioma: En Ano de publicação: 2017 Tipo de documento: Article