Synthesis of a biological active tumor growth factor from the predicted DNA sequence of Shope fibroma virus.
Biochemistry
; 27(15): 5640-5, 1988 Jul 26.
Article
em En
| MEDLINE
| ID: mdl-2846046
ABSTRACT
A 55-residue peptide comprising the carboxyl portion (residues 26-80) of the Shope fibroma virus growth factor (SFGF), a predicted 80-residue DNA virus gene product that encoded a homologous sequence with the epidermal growth factor transforming growth factor alpha family, was synthesized by a stepwise solid-phase method. The synthetic SFGF (26-80) purified to homogeneity by reverse-phase HPLC was characterized by fission ionization mass spectrometry and amino acid analysis. The disulfide pairings were established by enzymatic digestion and mass spectrometry and were found to be similar to those of EGF and TGF alpha. Synthetic SFGF (26-80) was found to share about 10% of the activities as EGF in the radioreceptor binding to A431 cells, stimulation of [3H]thymidine uptake in NRK cells, and induction of colony formation in soft-agar assay. Our results therefore confirmed that SFGF contained the putative biological activities of the EGF-TGF alpha family and that production of SFGF by Shope fibroma virus infected cells may account for the proliferative diseases associated with this particular virus.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poxviridae
/
Substâncias de Crescimento
/
Vírus do Fibroma dos Coelhos
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article