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Recombinant ostreolysin induces brown fat-like phenotype in HIB-1B cells.
Oren, Tom; Nimri, Lili; Yehuda-Shnaidman, Einav; Staikin, Katy; Hadar, Yitzhak; Friedler, Assaf; Amartely, Hadar; Slutzki, Michal; Pizio, Antonella Di; Niv, Masha Y; Peri, Irena; Graeve, Lutz; Schwartz, Betty.
Afiliação
  • Oren T; School of Nutritional Sciences, Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
  • Nimri L; School of Nutritional Sciences, Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
  • Yehuda-Shnaidman E; School of Nutritional Sciences, Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
  • Staikin K; School of Nutritional Sciences, Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
  • Hadar Y; Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.
  • Friedler A; Institute of Chemistry, the Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem, Israel.
  • Amartely H; Institute of Chemistry, the Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem, Israel.
  • Slutzki M; Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.
  • Pizio AD; Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.
  • Niv MY; Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.
  • Peri I; School of Nutritional Sciences, Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
  • Graeve L; Institute of Biological Chemistry and Nutrition, University of Hohenheim, Stuttgart, Germany.
  • Schwartz B; School of Nutritional Sciences, Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
Mol Nutr Food Res ; 61(9)2017 09.
Article em En | MEDLINE | ID: mdl-28464422
SCOPE: Brown adipose tissue (BAT) is the main regulator of thermogenesis by increasing energy expenditure through the uncoupling of oxidative metabolism from ATP synthesis. There is a growing body of evidence for BAT being the key responsible organ in combating obesity and its related disorders. Herein we propose the fungal protein ostreolysin (Oly), which has been previously shown to bind to cholesterol-enriched raft-like membrane domains (lipid rafts) of mammalian cells, as a suitable candidate for interaction with brown preadipocytes. The aim of the present study was therefore to characterize the mechanism by which a recombinant version of ostreolysin (rOly) induces brown adipocyte differentiation. METHODS AND RESULTS: Primary isolated brown preadipocytes or HIB-1B brown preadipocyte cells were treated with rOly and the effects on morphology, lipid accumulation, respiration rate, and associated gene and protein expression were measured. rOly upregulated mRNA and protein levels of factors related to brown adipocyte differentiation, induced lipid droplet formation, and increased cellular respiration rate due to expression of uncoupling protein 1. rOly also upregulated ß-tubulin expression, and therefore microtubules might be involved in its mechanism of action. CONCLUSION: rOly promotes brown adipocyte differentiation, suggesting a new mechanism for rOly's contribution to the battle against obesity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adipócitos Marrons / Proteínas Hemolisinas Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adipócitos Marrons / Proteínas Hemolisinas Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article