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Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution.
Fletcher, Russell B; Das, Diya; Gadye, Levi; Street, Kelly N; Baudhuin, Ariane; Wagner, Allon; Cole, Michael B; Flores, Quetzal; Choi, Yoon Gi; Yosef, Nir; Purdom, Elizabeth; Dudoit, Sandrine; Risso, Davide; Ngai, John.
Afiliação
  • Fletcher RB; Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
  • Das D; Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
  • Gadye L; Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, USA.
  • Street KN; Division of Biostatistics, University of California, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, CA 94720, USA.
  • Baudhuin A; Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
  • Wagner A; Department of Electrical Engineering and Computer Science, University of California, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, CA 94720, USA.
  • Cole MB; Department of Physics, University of California, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, CA 94720, USA.
  • Flores Q; Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
  • Choi YG; QB3 Functional Genomics Laboratory, University of California, Berkeley, CA 94720, USA.
  • Yosef N; Department of Electrical Engineering and Computer Science, University of California, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, CA 94720, USA.
  • Purdom E; Department of Statistics, University of California, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, CA 94720, USA.
  • Dudoit S; Division of Biostatistics, University of California, Berkeley, CA 94720, USA; Department of Statistics, University of California, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, CA 94720, USA.
  • Risso D; Division of Biostatistics, University of California, Berkeley, CA 94720, USA.
  • Ngai J; Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA; Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, USA; QB3 Functional Genomics Laboratory, University of California, Berkeley, CA 94720, USA. Electronic address: jngai@berkele
Cell Stem Cell ; 20(6): 817-830.e8, 2017 06 01.
Article em En | MEDLINE | ID: mdl-28506465
A detailed understanding of the paths that stem cells traverse to generate mature progeny is vital for elucidating the mechanisms governing cell fate decisions and tissue homeostasis. Adult stem cells maintain and regenerate multiple mature cell lineages in the olfactory epithelium. Here we integrate single-cell RNA sequencing and robust statistical analyses with in vivo lineage tracing to define a detailed map of the postnatal olfactory epithelium, revealing cell fate potentials and branchpoints in olfactory stem cell lineage trajectories. Olfactory stem cells produce support cells via direct fate conversion in the absence of cell division, and their multipotency at the population level reflects collective unipotent cell fate decisions by single stem cells. We further demonstrate that Wnt signaling regulates stem cell fate by promoting neuronal fate choices. This integrated approach reveals the mechanisms guiding olfactory lineage trajectories and provides a model for deconstructing similar hierarchies in other stem cell niches.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Divisão Celular / Mucosa Olfatória / Células-Tronco Multipotentes / Células-Tronco Adultas / Via de Sinalização Wnt Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Divisão Celular / Mucosa Olfatória / Células-Tronco Multipotentes / Células-Tronco Adultas / Via de Sinalização Wnt Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article