Polysaccharides from ginseng leaves inhibit tumor metastasis via macrophage and NK cell activation.
Int J Biol Macromol
; 103: 1327-1333, 2017 Oct.
Article
em En
| MEDLINE
| ID: mdl-28522391
ABSTRACT
In this study, we purified the polysaccharide fraction (GS-P) from the leaves of Panax ginseng C.A. Meyer and analyzed its monosaccharide composition and antitumor and antimetastatic activity in vitro and in vivo. GS-P is a 10.2kDa pectic polysaccharide consisting of 15 different monosaccharides. GS-P treatment significantly inhibited metastasis in mice, in a dose-dependent manner. GS-P was not cytotoxic to colon 26-M3.1 cells and increased mouse splenocyte proliferation. Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice. Moreover, PEMs obtained from GS-P-treated mice showed significantly higher tumoricidal activity against colon 26-M3.1 cells, and splenocytes from GS-P-treated mice significantly enhanced NK cell cytotoxicity against YAC-1 tumor cells. Pretreatment with anti-asialo GM1 (an antibody for NK cell depletion) partly suppressed the inhibitory effects of GS-P on lung metastasis. These data suggest that GS-P exhibits antimetastatic activity by promoting the activation of macrophages and NK cells.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Células Matadoras Naturais
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Ativação Linfocitária
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Folhas de Planta
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Panax
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Ativação de Macrófagos
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Macrófagos
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article