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Shifting the optimal stiffness for cell migration.
Bangasser, Benjamin L; Shamsan, Ghaidan A; Chan, Clarence E; Opoku, Kwaku N; Tüzel, Erkan; Schlichtmann, Benjamin W; Kasim, Jesse A; Fuller, Benjamin J; McCullough, Brannon R; Rosenfeld, Steven S; Odde, David J.
Afiliação
  • Bangasser BL; Department of Biomedical Engineering, University of Minnesota, 312 Church Street SE, Minneapolis, Minnesota 55455, USA.
  • Shamsan GA; Department of Biomedical Engineering, University of Minnesota, 312 Church Street SE, Minneapolis, Minnesota 55455, USA.
  • Chan CE; Department of Biomedical Engineering, University of Minnesota, 312 Church Street SE, Minneapolis, Minnesota 55455, USA.
  • Opoku KN; Department of Biomedical Engineering, University of Minnesota, 312 Church Street SE, Minneapolis, Minnesota 55455, USA.
  • Tüzel E; Department of Biomedical Engineering, University of Minnesota, 312 Church Street SE, Minneapolis, Minnesota 55455, USA.
  • Schlichtmann BW; Department of Biomedical Engineering, University of Minnesota, 312 Church Street SE, Minneapolis, Minnesota 55455, USA.
  • Kasim JA; Department of Biomedical Engineering, University of Minnesota, 312 Church Street SE, Minneapolis, Minnesota 55455, USA.
  • Fuller BJ; Department of Biomedical Engineering, University of Minnesota, 312 Church Street SE, Minneapolis, Minnesota 55455, USA.
  • McCullough BR; Department of Biomedical Engineering, University of Minnesota, 312 Church Street SE, Minneapolis, Minnesota 55455, USA.
  • Rosenfeld SS; Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA.
  • Odde DJ; Department of Biomedical Engineering, University of Minnesota, 312 Church Street SE, Minneapolis, Minnesota 55455, USA.
Nat Commun ; 8: 15313, 2017 05 22.
Article em En | MEDLINE | ID: mdl-28530245
ABSTRACT
Cell migration, which is central to many biological processes including wound healing and cancer progression, is sensitive to environmental stiffness, and many cell types exhibit a stiffness optimum, at which migration is maximal. Here we present a cell migration simulator that predicts a stiffness optimum that can be shifted by altering the number of active molecular motors and clutches. This prediction is verified experimentally by comparing cell traction and F-actin retrograde flow for two cell types with differing amounts of active motors and clutches embryonic chick forebrain neurons (ECFNs; optimum ∼1 kPa) and U251 glioma cells (optimum ∼100 kPa). In addition, the model predicts, and experiments confirm, that the stiffness optimum of U251 glioma cell migration, morphology and F-actin retrograde flow rate can be shifted to lower stiffness by simultaneous drug inhibition of myosin II motors and integrin-mediated adhesions.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Movimento Celular / Actinas / Prosencéfalo / Glioma / Neurônios Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Movimento Celular / Actinas / Prosencéfalo / Glioma / Neurônios Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article