Versatility of a vector for expressing foreign polypeptides at the surface of gram-negative bacteria.
Gene
; 70(1): 181-9, 1988 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-2853688
ABSTRACT
A wide variety of peptides in terms of length and sequence can be expressed at the surface of the bacterium Escherichia coli by genetic insertion into a 'permissive' site of the outer membrane protein LamB, used as a carrier. The resulting hybrid proteins essentially keep their biological activities with inserts of up to about 60 amino acid residues, and of a large range of predicted structures or hydrophobicities. This reflects a remarkable flexibility in the organization of the protein, but also in the export machinery. The method used to select such a permissive site is quite general and its potential to generate applications, including a versatile type of live bacterial vaccine, are discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Plasmídeos
/
Vetores Genéticos
/
Bactérias Gram-Negativas
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article