Application of molecular, microbiological, and immunological tests for the diagnosis of bone and joint tuberculosis.
J Clin Lab Anal
; 32(2)2018 Feb.
Article
em En
| MEDLINE
| ID: mdl-28543876
ABSTRACT
BACKGROUND:
To evaluate the application of interferon gamma release assay (IGRA), rifampicin resistant real-time fluorescence quantitative PCR technique Xpert Mycobacterium tuberculosis/rifampicin (Xpert MTB/RIF), and the levels of TNF-α and TGF-ß in the diagnosis of bone and joint tuberculosis.METHODS:
Eighty-six patients with bone and joint tuberculosis, diagnosed by pathology or microbiology, were examined by Xpert MTB/RIF and IGRA (T-SPOT. TB) for Mycobacterium tuberculosis infection, and the TNF-α and TGF-ß levels of the patients were measured.RESULTS:
The sensitivity of IGRA in diagnosing bone and joint tuberculosis was 81.4%; Xpert MTB/RIF's sensitivity was 70.9%. The combined sensitivity of the two methods was 91.9%. The combined detection sensitivity of the two methods was higher than individual IGRA or Xpert MTB/RIF detection sensitivity. The TNF-α and TGF-ß levels in bone and joint tuberculosis patients were higher than those in the control group.CONCLUSION:
Xpert MTB/RIF, IGRA, TNF-α, and TGF-ßs expression have value in the rapid diagnosis of bone and joint tuberculosis, and the sensitivity and accuracy of bone and joint tuberculosis diagnosis by combining them can improve it.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tuberculose Osteoarticular
/
Tipagem Molecular
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Mycobacterium tuberculosis
Tipo de estudo:
Diagnostic_studies
Limite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article