Detection of herpes simplex virus by biotinylated DNA probes.
Diagn Microbiol Infect Dis
; 11(3): 145-9, 1988 Nov.
Article
em En
| MEDLINE
| ID: mdl-2854512
ABSTRACT
Clinical specimens from 159 patients suspected with herpes simplex virus (HSV) were examined by monoclonal antibody immunofluorescence (IF) and by a commercial biotinylated DNA probe kit following cell culture isolation. Herpes simplex virus was isolated from 57 samples. All cultures were positive by IF when the cytopathic effect (CPE) was less than 1+ but only 49 (86%) yielded positive reaction with the DNA probe when CPE was at least 1+. A total of 54 clinical specimens was also examined directly by immunoperoxidase histopathology (IHP), IF, and DNA hybridization. Of these, 16 were positive by IHP, 15 by IF, and only five by DNA probe. The DNA probe kit was found to be reasonably sensitive only after cell culture isolation of HSV. Compared to the IF procedure, the DNA probe kit was found to be costly, labor intensive, and time consuming.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sondas de DNA
/
Simplexvirus
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article