A novel immuno-gold labeling protocol for nanobody-based detection of HER2 in breast cancer cells using immuno-electron microscopy.

Kijanka, M; van Donselaar, E G; Müller, W H; Dorresteijn, B; Popov-Celeketic, D; El Khattabi, M; Verrips, C T; van Bergen En Henegouwen, P M P; Post, J A

Kijanka, M; van Donselaar, E G; Müller, W H; Dorresteijn, B; Popov-Celeketic, D; El Khattabi, M; Verrips, C T; van Bergen En Henegouwen, P M P; Post, J A.

Afiliação

Kijanka M; Division of Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.
van Donselaar EG; Department of Cell Biology, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht, The Netherlands.
Müller WH; Cryo-EM, Department of Chemistry, Faculty of Science, Utrecht University, Utrecht, The Netherlands.
Dorresteijn B; Division of Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.
Popov-Celeketic D; Division of Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.
El Khattabi M; QVQ Holding BV, Utrecht, The Netherlands.
Verrips CT; QVQ Holding BV, Utrecht, The Netherlands.
van Bergen En Henegouwen PMP; Division of Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands. Electronic address: p.vanbergen@uu.nl.
Post JA; Division of Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.

J Struct Biol ; 199(1): 1-11, 2017 07.

Article em En | MEDLINE | ID: mdl-28552722

Assuntos

Neoplasias da Mama/diagnóstico por imagem; Microscopia Imunoeletrônica/métodos; Receptor ErbB-2/análise; Anticorpos de Domínio Único/imunologia; Fixação de Tecidos/métodos; Animais; Ouro; Humanos; Microscopia Imunoeletrônica/normas; Receptor ErbB-2/imunologia; Projetos de Pesquisa; Coloração e Rotulagem/normas; Fixação de Tecidos/normas

Palavras-chave

Electron microscopy; HER2; Light microscopy; Nanobody; SEM; TEM; VHH

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Microscopia Imunoeletrônica / Fixação de Tecidos / Receptor ErbB-2 / Anticorpos de Domínio Único Tipo de estudo: Diagnostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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