Vancomycin-Dependent Response in Live Drug-Resistant Bacteria by Metabolic Labeling.
Angew Chem Int Ed Engl
; 56(30): 8839-8843, 2017 07 17.
Article
em En
| MEDLINE
| ID: mdl-28570012
ABSTRACT
The surge in drug-resistant bacterial infections threatens to overburden healthcare systems worldwide. Bacterial cell walls are essential to bacteria, thus making them unique targets for the development of antibiotics. We describe a cellular reporter to directly monitor the phenotypic switch in drug-resistant bacteria with temporal resolution. Vancomycin-resistant enterococci (VRE) escape the bactericidal action of vancomycin by chemically modifying their cell-wall precursors. A synthetic cell-wall analogue was developed to hijack the biosynthetic rewiring of drug-resistant cells in response to antibiotics. Our study provides the first in vivo VanX reporter agent that responds to cell-wall alteration in drug-resistant bacteria. Cellular reporters that reveal mechanisms related to antibiotic resistance can potentially have a significant impact on the fundamental understanding of cellular adaption to antibiotics.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Resistência Microbiana a Medicamentos
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Vancomicina
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Enterococcus faecium
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Antibacterianos
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article