Anti-inflammatory effects and mechanisms of Hizikia fusiformis via multicellular signaling pathways in lipopolysaccharide-induced RAW 264.7 cells.
Pak J Pharm Sci
; 30(1): 43-48, 2017 Jan.
Article
em En
| MEDLINE
| ID: mdl-28603111
ABSTRACT
In this study, we investigated the anti-inflammatory effects and mechanisms of Hizikia fusiformis (HF) extracts in lipopolysaccharide (LPS)-induced RAW 264.7 cells. We extracted HF using solvent and sub-critical water techniques. In results, HF extracts inhibited nitric oxide (NO) production in cell-free and LPS-stimulated RAW 264.7 cells. HF210 (extract prepared with sub critical water at 210oC) was most effective. The HF210 extract dose-dependently inhibited inducible nitric oxide synthase expression (iNOS) and nuclear factor kappa (NF-ï«B) p65 translocation from cytosol to the nucleus. Furthermore, HF210 extract dose-dependently inhibited the phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK), Jun N-terminal kinase (JNK), and signal transducers and activators of transcription (STAT)-1in LPS-induced RAW 264.7 cells. Thus, our results suggest that anti-inflammatory effects of HF210 extract showed a noticeable distinction by regulation of multiple signaling pathways in LPS-induced RAW 264.7 cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Alga Marinha
/
Transdução de Sinais
/
Lipopolissacarídeos
/
Inflamação
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Macrófagos
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Anti-Inflamatórios
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article