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Age and Gender Associations of Virus Positivity in Merkel Cell Carcinoma Characterized Using a Novel RNA In Situ Hybridization Assay.
Wang, Lisha; Harms, Paul W; Palanisamy, Nallasivam; Carskadon, Shannon; Cao, Xuhong; Siddiqui, Javed; Patel, Rajiv M; Zelenka-Wang, Sylvia; Durham, Alison B; Fullen, Douglas R; Harms, Kelly L; Su, Fengyun; Shukla, Sudhanshu; Mehra, Rohit; Chinnaiyan, Arul M.
Afiliação
  • Wang L; Michigan Center for Translational Pathology, University of Michigan Health System, Ann Arbor, Michigan.
  • Harms PW; Michigan Center for Translational Pathology, University of Michigan Health System, Ann Arbor, Michigan.
  • Palanisamy N; Department of Pathology, University of Michigan Health System, Ann Arbor, Michigan.
  • Carskadon S; Department of Dermatology, University of Michigan Health System, Ann Arbor, Michigan.
  • Cao X; Michigan Center for Translational Pathology, University of Michigan Health System, Ann Arbor, Michigan.
  • Siddiqui J; Department of Pathology, University of Michigan Health System, Ann Arbor, Michigan.
  • Patel RM; Department of Urology, Henry Ford Health System, Detroit, Michigan.
  • Zelenka-Wang S; Department of Urology, Henry Ford Health System, Detroit, Michigan.
  • Durham AB; Michigan Center for Translational Pathology, University of Michigan Health System, Ann Arbor, Michigan.
  • Fullen DR; Howard Hughes Medical Institute, Ann Arbor, Michigan.
  • Harms KL; Michigan Center for Translational Pathology, University of Michigan Health System, Ann Arbor, Michigan.
  • Su F; Department of Pathology, University of Michigan Health System, Ann Arbor, Michigan.
  • Shukla S; Department of Pathology, University of Michigan Health System, Ann Arbor, Michigan.
  • Mehra R; Department of Dermatology, University of Michigan Health System, Ann Arbor, Michigan.
  • Chinnaiyan AM; Michigan Center for Translational Pathology, University of Michigan Health System, Ann Arbor, Michigan.
Clin Cancer Res ; 23(18): 5622-5630, 2017 Sep 15.
Article em En | MEDLINE | ID: mdl-28606924
ABSTRACT

Purpose:

Merkel cell carcinoma (MCC) is a highly aggressive neuroendocrine tumor of the skin. Merkel cell polyomavirus (MCPyV) plays an oncogenic role in the majority of MCCs. Detection of MCPyV in MCCs has diagnostic utility and prognostic potential. We investigated whether RNAscope, an RNA in situ hybridization (ISH) assay for detection of RNA transcripts in tissues, is useful for MCPyV detection.Experimental

Design:

We applied an RNAscope probe targeting MCPyV T antigen transcripts on tissue microarrays (TMA) and whole-tissue sections encompassing 87 MCCs from 75 patients, 14 carcinomas of other types, and benign tissues. For comparison, qPCR was performed on 57 cases of MCC from 52 patients.

Results:

RNA-ISH demonstrated the presence of MCPyV in 37 of 75 cases (49.3%). Notably, tumors from younger patients (<73 years) had a significantly higher virus positivity than those from elderly patients (≥73 years; 64.9% vs. 34.2%, P = 0.011). Female patients had a higher positive rate of MCPyV than male patients (66.7% vs. 39.6%, P = 0.032). Data from both RNA-ISH and qPCR were available for 57 samples. Considering MCPyV qPCR as the gold standard for determining MCPyV status, RNAscope had 100% sensitivity and 100% specificity. There was a strong correlation between qPCR copy number and RNA-ISH product score (Spearman correlation coefficient R2 = 0.932, P < 0.0001).

Conclusions:

RNA-ISH is comparably sensitive to qPCR for detection of MCPyV and allows for correlation with tissue morphology. This study also reveals a significant association between age, gender, and MCPyV positivity. Clin Cancer Res; 23(18); 5622-30. ©2017 AACR.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Tumorais por Vírus / Carcinoma de Célula de Merkel / Hibridização In Situ Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Tumorais por Vírus / Carcinoma de Célula de Merkel / Hibridização In Situ Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2017 Tipo de documento: Article