Synthetic ligand promotes gene expression by affecting GC sequence in promoter.
Bioorg Med Chem Lett
; 27(15): 3391-3394, 2017 08 01.
Article
em En
| MEDLINE
| ID: mdl-28610980
ABSTRACT
A naphthyridine carbamate tetramer (NCT8) is a synthetic compound, which selectively binds to nucleic acids containing CGG/CGG sequence. Although NCT8 is a promising compound for a wide range of DNA and RNA based biotechnology such as modulation of specific gene expression, little is known about its behavior in human cells. In the present study, we investigated the changes induced in gene expression by NCT8. Genes differentially expressed in the presence of NCT8 in HeLa cells were identified by whole-transcriptome analysis. The whole-transcriptome analysis showed that NCT8 significantly induced up-regulation of specific genes, whose promoter region has GC-rich sequence.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carbamatos
/
Regulação para Cima
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Regiões Promotoras Genéticas
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Sequência Rica em GC
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Naftiridinas
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article