Single-molecule Förster resonance energy transfer (FRET) analysis discloses the dynamics of the DNA-topoisomerase II (Top2) interaction in the presence of TOP2-targeting agents.

Huang, Wan-Chen; Lee, Chun-Ying; Hsieh, Tao-Shih

Huang, Wan-Chen; Lee, Chun-Ying; Hsieh, Tao-Shih.

Afiliação

Huang WC; Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 115, Taiwan. Electronic address: wchuang1@gate.sinica.edu.tw.
Lee CY; Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 115, Taiwan; Department of Chemistry, National Taiwan University, Taipei 106, Taiwan.
Hsieh TS; Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 115, Taiwan; Department of Chemistry, National Taiwan University, Taipei 106, Taiwan; Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.

J Biol Chem ; 292(30): 12589-12598, 2017 07 28.

Article em En | MEDLINE | ID: mdl-28630044

Assuntos

DNA Topoisomerases Tipo II/metabolismo; Drosophila melanogaster/enzimologia; Transferência Ressonante de Energia de Fluorescência; Animais; Antineoplásicos/farmacologia; DNA/química; DNA/metabolismo; DNA Topoisomerases Tipo II/química; Dexrazoxano/farmacologia; Transferência de Energia; Relação Estrutura-Atividade

Palavras-chave

DNA gate dynamics; DNA topoisomerase; anticancer drug; enzyme kinetics; protein dynamic; protein-DNA interaction; single-molecule Forster Resonance Energy Transfer; single-molecule biophysics

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Topoisomerases Tipo II / Transferência Ressonante de Energia de Fluorescência / Drosophila melanogaster Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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