Enhanced simultaneous overlap extension-PCR by gold nanoparticles.
Nanomedicine
; 13(7): 2263-2266, 2017 Oct.
Article
em En
| MEDLINE
| ID: mdl-28673853
ABSTRACT
Methods to fuse multiple DNA fragments are extremely useful in synthetic biology and protein engineering. Here, we report a gold nanoparticle-mediated simultaneous overlap extension-PCR (AuNP-mediated SOE-PCR) method that enables the fusion of multiple DNA fragments simultaneously with their amplification in a single reaction using typical PCR conditions. Using greater concentrations of rTaq DNA polymerase and AuNPs significantly improves the performance of SOE-PCR especially for the fusion of more than three DNA fragments. We show that up to six lambda DNA fragments can be simultaneously fused by AuNP-mediated SOE-PCR.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
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Reação em Cadeia da Polimerase
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Nanopartículas Metálicas
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Ouro
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article