Improving bioassay sensitivity through immobilization of bio-probes onto reactive micelles.
Chem Commun (Camb)
; 53(57): 8062-8065, 2017 Jul 13.
Article
em En
| MEDLINE
| ID: mdl-28674711
An antigen probe (HIV-1 p24) immobilized onto N-succinimidyl ester based micelles was used as a solid phase coating in ELISA test, and induced a significant improvement in antibody detection sensitivity as compared to the standard free antigen coating. The relevance of this straightforward approach to improve the bioassay sensitivity was confirmed by using biotin as a generic probe.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biotina
/
Ensaio de Imunoadsorção Enzimática
/
Sondas Moleculares
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Proteína do Núcleo p24 do HIV
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Micelas
/
Anticorpos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article