Imaging phospholipid conformational disorder and packing in giant multilamellar liposome by confocal Raman microspectroscopy.
Spectrochim Acta A Mol Biomol Spectrosc
; 187: 186-190, 2017 Dec 05.
Article
em En
| MEDLINE
| ID: mdl-28689162
ABSTRACT
Liposomes are closed phospholipid bilayer systems that have profound applications in fundamental cell biology, pharmaceutics and medicine. Depending on the composition (pure or mixture of phospholipids, presence of cholesterol) and preparation protocol, intra- and inter-chain molecular interactions vary leading to changes in the quality (order and packing) of liposomes. So far it is not possible to image conformational disorders and packing densities within a liposome in a straightforward manner. In this study, we utilized confocal Raman microspectroscopy to visualize structural disorders and packing efficiency within a giant multilamellar liposome model by focusing mainly on three regions in the vibrational spectrum (CC stretching, CH deformation and CH stretching). We estimated properties such as trans/gauche isomers and lateral packing probability. Interestingly, our Raman imaging studies revealed gel phase rich domains and heterogeneous lateral packing within the giant multilamellar liposome.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfolipídeos
/
Análise Espectral Raman
/
Lipossomos
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article