A short synthetic chimeric sequence harboring matrix attachment region/PSAR2 increases transgene expression in Chinese hamster ovary cells.
Biosci Biotechnol Biochem
; 81(9): 1755-1761, 2017 Sep.
Article
em En
| MEDLINE
| ID: mdl-28715274
ABSTRACT
A chimeric DNA fragment containing an interferon-beta matrix attachment region (MAR) and an immunoglobulin MAR (PSAR2) was synthesized. PSAR2 was cloned into the upstream or downstream region of an enhanced green fluorescent protein (eGFP) expression cassette in a eukaryotic vector, which was then transfected into CHO cells. The results showed that PSAR2 did not effectively increase transgene expression when it was cloned into the upstream region of the eGFP expression cassette. However, when inserted downstream of the eGFP expression cassette, PSAR2-enhanced transient transgene expression and significantly increased the numbers of stably transfected cells compared with the control vector. Additionally, PSAR2 significantly increased eGFP copy numbers as compared with the control vector. PSAR2 could significantly enhance transgene expression in CHO cells according to the position in the vector and increased transgene copy numbers. We found a short chimeric sequence harboring two MARs effectively increased transgene expression in CHO cells.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulinas
/
Engenharia Genética
/
Transgenes
/
Regiões de Interação com a Matriz
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article