TGF-ß signaling regulates p-Akt levels via PP2A during diapause entry in the cotton bollworm, Helicoverpa armigera.
Insect Biochem Mol Biol
; 87: 165-173, 2017 08.
Article
em En
| MEDLINE
| ID: mdl-28720534
ABSTRACT
Akt, which is a key kinase in the insulin signaling pathway, plays important roles in glucose metabolism, cell proliferation, transcription and cell migration. Our previous studies indicated that low insulin levels and high p-Akt levels are present in diapause-destined individuals. Here, we show that PI3K, which is upstream of Akt, is low in diapause-destined pupal brains but high in p-Akt levels, implying that p-Akt is modified by factors other than the insulin signaling pathway. Protein phosphatase 2A (PP2A), which is a key regulator in the TGF-ß signaling pathway, can directly bind to and dephosphorylate Akt. Low PP2A expression and activity in diapause-destined individuals suggest that a weak Akt dephosphorylation contributes to p-Akt accumulation. In addition, transforming growth factor-ß receptor I (TßRI), which is upstream of PP2A, increases the activity of PP2A and decreases the p-Akt levels. These results show that TGF-ß signaling decreases p-Akt levels by increasing the activity of PP2A. This is the first report showing that TGF-ß signaling negatively regulates the insulin pathway in insect development or diapause.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fator de Crescimento Transformador beta
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Proteínas Proto-Oncogênicas c-akt
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Proteína Fosfatase 2
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Diapausa de Inseto
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Mariposas
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article