Cyramza induces apoptosis of HCC4006 cell by affecting the level of Bcl-w.
Eur Rev Med Pharmacol Sci
; 21(13): 3069-3074, 2017 Jul.
Article
em En
| MEDLINE
| ID: mdl-28742199
ABSTRACT
OBJECTIVE:
Lung cancer seriously threats to patient's life and health. Cyramza is a therapeutic drug for inhibition of vessel formation and growth in clinical practice. The aim of this study was to investigate the effect of cyramza on growth and apoptosis of non-small lung cancer HCC4006 and explore the related mechanisms. MATERIALS ANDMETHODS:
Cell viability and apoptosis were examined by MTT assay and flow cytometry, respectively. Western blot was employed to examine the effect of cyramza on the apoptotic protein Bcl-w. After that Bcl-w knockdown and overexpression were respectively fulfilled by Bcl-w siRNA and plasmid transfection, effects of cyramza on cell apoptosis were determined by Western-blot.RESULTS:
Cyramza inhibited the cell growth and induced the cell apoptosis in HCC4006 cells, which was mediated by downregulation of Bcl-w level. Bcl-w knockdown and overexpression could increase and decrease the effect of cyramza on cell apoptosis, respectively.CONCLUSIONS:
Cyramza induced the apoptosis of non-small lung cancer cell line HCC4006 via the downregulation of Bcl-w.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apoptose
/
Proteínas Reguladoras de Apoptose
/
Anticorpos Monoclonais
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article