Improved sensitivity of firefly luminescent intermediate-based protein interaction assay using Ser 440 mutant with lower adenylation activity.

Ohmuro-Matsuyama, Yuki; Yamashita, Takahiro; Lin, Huan; Yamaji, Hideki; Ueda, Hiroshi

Ohmuro-Matsuyama, Yuki; Yamashita, Takahiro; Lin, Huan; Yamaji, Hideki; Ueda, Hiroshi.

Afiliação

Ohmuro-Matsuyama Y; Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Midori-ku, Yokohama, Japan.
Yamashita T; Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Nada-ku, Kobe, Japan.
Lin H; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Yamaji H; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Ueda H; Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Nada-ku, Kobe, Japan.

Luminescence ; 33(1): 125-130, 2018 Feb.

Article em En | MEDLINE | ID: mdl-28752574

Assuntos

Luciferases de Vaga-Lume/química; Luminescência; Serina/genética; Animais; Vaga-Lumes; Luciferases de Vaga-Lume/genética; Luciferases de Vaga-Lume/metabolismo; Mutação; Ligação Proteica; Serina/metabolismo

Palavras-chave

conformational change; firefly luciferase; protein-protein interaction

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Serina / Luciferases de Vaga-Lume / Luminescência Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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