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A multiplexed screening method for pluripotency.
Plotnikov, Alexander; Kozer, Noga; Krupalnik, Vladislav; Peles, Shani; Mor, Nofar; Rais, Yoach; Hanna, Jacob H; Barr, Haim M.
Afiliação
  • Plotnikov A; The Nancy & Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel.
  • Kozer N; The Nancy & Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel.
  • Krupalnik V; The Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
  • Peles S; The Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
  • Mor N; The Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
  • Rais Y; The Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
  • Hanna JH; The Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
  • Barr HM; The Nancy & Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel. Electronic address: haim.barr@weizmann.ac.il.
Stem Cell Res ; 23: 158-162, 2017 08.
Article em En | MEDLINE | ID: mdl-28756340
ABSTRACT
Measurement of Alkaline Phosphatase (ALP) level is a widely used procedure in clinical and basic research. We present a simple and inexpensive luminescence-based method that allows multiplexed measurement and normalization of intracellular ALP levels in one sample well. The method comprises two commercially available reagents enabling quantification of ALP levels and cell number by two sequential luminescence readouts. Using this method we were able to detect and analyze somatic reprogramming into pluripotent stem cells. The method is highly applicable for High Throughput Screening (HTS) campaigns and analysis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes Induzidas / Medições Luminescentes Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes Induzidas / Medições Luminescentes Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article