Peptide-Membrane Interaction between Targeting and Lysis.
ACS Chem Biol
; 12(9): 2254-2259, 2017 09 15.
Article
em En
| MEDLINE
| ID: mdl-28763193
ABSTRACT
Certain cationic peptides interact with biological membranes. These often-complex interactions can result in peptide targeting to the membrane, or in membrane permeation, rupture, and cell lysis. We investigated the relationship between the structural features of membrane-active peptides and these effects, to better understand these processes. To this end, we employed a computational method for morphing a membranolytic antimicrobial peptide into a nonmembranolytic mitochondrial targeting peptide by "directed simulated evolution." The results obtained demonstrate that superficially subtle sequence modifications can strongly affect the peptides' membranolytic and membrane-targeting abilities. Spectroscopic and computational analyses suggest that N- and C-terminal structural flexibility plays a crucial role in determining the mode of peptide-membrane interaction.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Staphylococcus aureus
/
Peptídeos Catiônicos Antimicrobianos
/
Lipossomos
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Anti-Infecciosos
/
Mitocôndrias
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article