Dual RNA Processing Roles of Pat1b via Cytoplasmic Lsm1-7 and Nuclear Lsm2-8 Complexes.
Cell Rep
; 20(5): 1187-1200, 2017 08 01.
Article
em En
| MEDLINE
| ID: mdl-28768202
ABSTRACT
Pat1 RNA-binding proteins, enriched in processing bodies (P bodies), are key players in cytoplasmic 5' to 3' mRNA decay, activating decapping of mRNA in complex with the Lsm1-7 heptamer. Using co-immunoprecipitation and immunofluorescence approaches coupled with RNAi, we provide evidence for a nuclear complex of Pat1b with the Lsm2-8 heptamer, which binds to the spliceosomal U6 small nuclear RNA (snRNA). Furthermore, we establish the set of interactions connecting Pat1b/Lsm2-8/U6 snRNA/SART3 and additional U4/U6.U5 tri-small nuclear ribonucleoprotein particle (tri-snRNP) components in Cajal bodies, the site of snRNP biogenesis. RNA sequencing following Pat1b depletion revealed the preferential upregulation of mRNAs normally found in P bodies and enriched in 3' UTR AU-rich elements. Changes in >180 alternative splicing events were also observed, characterized by skipping of regulated exons with weak donor sites. Our data demonstrate the dual role of a decapping enhancer in pre-mRNA processing as well as in mRNA decay via distinct nuclear and cytoplasmic Lsm complexes.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Precursores de RNA
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Processamento Pós-Transcricional do RNA
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Proteínas Proto-Oncogênicas
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Proteínas de Ligação a RNA
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Ribonucleoproteína Nuclear Pequena U4-U6
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Ribonucleoproteínas Nucleares Pequenas
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Complexos Multiproteicos
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Proteínas de Ligação a DNA
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Acetiltransferase N-Terminal C
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article