Neutral Red versus MTT assay of cell viability in the presence of copper compounds.
Anal Biochem
; 535: 43-46, 2017 10 15.
Article
em En
| MEDLINE
| ID: mdl-28778493
ABSTRACT
Copper is essential for numerous physiological functions, and copper compounds may display therapeutic as well as cytotoxic effects. The MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assay is a standard test largely used in cytotoxicity studies. This report shows that low micromolar levels of copper compounds such as Cu(II)Urea2, Cu(II)Ser2 and CuCl2 can interfere with the MTT assay making improper the detection of formazan product of MTT reduction. Comparatively, the Neutral Red assay appears to be sensitive and showing no interference with these compounds. The lactate dehydrogenase alternative assay cannot be used because of inhibitory effect of these copper compounds on the enzyme activity.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Compostos Organometálicos
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Sais de Tetrazólio
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Bioensaio
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Cobre
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Vermelho Neutro
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article