Three-Dimensional Control of Ion Channel Function through Optogenetics and Co-Culture.
SLAS Discov
; 23(1): 102-108, 2018 01.
Article
em En
| MEDLINE
| ID: mdl-28783478
The lack of miniaturized and cost-effective methods to control cellular excitability with dosable and temporally precise electrical perturbations represents a long-lasting and unsolved bottleneck for ion channel drug discovery pipelines. Here we developed a high-throughput-compatible fluorescent-based cellular assay that combines optogenetics and co-culture approaches to obtain spatial, temporal, and quantitative control of ion channel activity. The modularity and increased flexibility of control of this light-tandem assay, combined with contained costs and compatibility with conventional drug-screening platforms, make this system suitable for temporally precise screening of ion channel function in controlled conformations and can also be used to recapitulate other complexly regulated biological processes.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Descoberta de Drogas
/
Ensaios de Triagem em Larga Escala
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Optogenética
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Canais Iônicos
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article