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Id genes are essential for early heart formation.
Cunningham, Thomas J; Yu, Michael S; McKeithan, Wesley L; Spiering, Sean; Carrette, Florent; Huang, Chun-Teng; Bushway, Paul J; Tierney, Matthew; Albini, Sonia; Giacca, Mauro; Mano, Miguel; Puri, Pier Lorenzo; Sacco, Alessandra; Ruiz-Lozano, Pilar; Riou, Jean-Francois; Umbhauer, Muriel; Duester, Gregg; Mercola, Mark; Colas, Alexandre R.
Afiliação
  • Cunningham TJ; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, 92037, USA.
  • Yu MS; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, 92037, USA.
  • McKeithan WL; Department of Bioengineering, University of California at San Diego, La Jolla, California 92037, USA.
  • Spiering S; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, 92037, USA.
  • Carrette F; Graduate School of Biomedical Sciences, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California 92037, USA.
  • Huang CT; Department of Medicine and Cardiovascular Institute, Stanford University, Palo Alto, California 94305, USA.
  • Bushway PJ; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, 92037, USA.
  • Tierney M; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, 92037, USA.
  • Albini S; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, 92037, USA.
  • Giacca M; Department of Bioengineering, University of California at San Diego, La Jolla, California 92037, USA.
  • Mano M; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, 92037, USA.
  • Puri PL; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, 92037, USA.
  • Sacco A; International Centre for Genetic Engineering and Biotechnology, 34149 Trieste, Italy.
  • Ruiz-Lozano P; Center for Neuroscience and Cell Biology (CNC), University of Coimbra, 3004-504 Coimbra, Portugal.
  • Riou JF; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, 92037, USA.
  • Umbhauer M; Istituti di Ricovero e Cura a Carattere Scientifico, Fondazione Santa Lucia, 00179 Rome, Italy.
  • Duester G; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, 92037, USA.
  • Mercola M; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, 92037, USA.
  • Colas AR; Regencor, Inc., Los Altos, California 94022, USA.
Genes Dev ; 31(13): 1325-1338, 2017 07 01.
Article em En | MEDLINE | ID: mdl-28794185
Deciphering the fundamental mechanisms controlling cardiac specification is critical for our understanding of how heart formation is initiated during embryonic development and for applying stem cell biology to regenerative medicine and disease modeling. Using systematic and unbiased functional screening approaches, we discovered that the Id family of helix-loop-helix proteins is both necessary and sufficient to direct cardiac mesoderm formation in frog embryos and human embryonic stem cells. Mechanistically, Id proteins specify cardiac cell fate by repressing two inhibitors of cardiogenic mesoderm formation-Tcf3 and Foxa2-and activating inducers Evx1, Grrp1, and Mesp1. Most importantly, CRISPR/Cas9-mediated ablation of the entire Id (Id1-4) family in mouse embryos leads to failure of anterior cardiac progenitor specification and the development of heartless embryos. Thus, Id proteins play a central and evolutionarily conserved role during heart formation and provide a novel means to efficiently produce cardiovascular progenitors for regenerative medicine and drug discovery applications.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linhagem da Célula / Organogênese / Proteínas Inibidoras de Diferenciação / Coração Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linhagem da Célula / Organogênese / Proteínas Inibidoras de Diferenciação / Coração Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article