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Automated synthesis of 1-[11C]acetoacetate on a TRASIS AIO module.
Solingapuram Sai, Kiran Kumar; Donald Gage, H; Almaguel, Frankis; Neth, Bryan; Hughes, Timothy M; Tremblay, Sebastien; Castellano, Christian-Alexandre; Cunnane, Stephen C; Jorgensen, Matthew J; Craft, Suzanne; Mintz, Akiva.
Afiliação
  • Solingapuram Sai KK; Department of Radiology, Wake Forest School of Medicine, Winston Salem, NC, USA. Electronic address: ksolinga@wakehealth.edu.
  • Donald Gage H; Department of Radiology, Wake Forest School of Medicine, Winston Salem, NC, USA.
  • Almaguel F; Department of Radiology, Wake Forest School of Medicine, Winston Salem, NC, USA.
  • Neth B; Department of Internal Medicine, Wake Forest School of Medicine, Winston Salem, NC, USA.
  • Hughes TM; Department of Internal Medicine, Wake Forest School of Medicine, Winston Salem, NC, USA.
  • Tremblay S; Research Center on Aging, Sherbrooke Molecular Imaging Center, Université de Sherbrooke, Sherbrooke, Quebec, Canada.
  • Castellano CA; Research Center on Aging, Sherbrooke Molecular Imaging Center, Université de Sherbrooke, Sherbrooke, Quebec, Canada.
  • Cunnane SC; Research Center on Aging, Sherbrooke Molecular Imaging Center, Université de Sherbrooke, Sherbrooke, Quebec, Canada.
  • Jorgensen MJ; Department of Pathology-Comparative Medicine, Wake Forest School of Medicine, USA.
  • Craft S; Department of Internal Medicine, Wake Forest School of Medicine, Winston Salem, NC, USA.
  • Mintz A; Department of Radiology, Wake Forest School of Medicine, Winston Salem, NC, USA.
Appl Radiat Isot ; 129: 57-61, 2017 Nov.
Article em En | MEDLINE | ID: mdl-28806598
ABSTRACT
We automated radiochemical synthesis of 1-[11C]acetoacetate in a commercially available radiochemistry module, TRASIS AllInOne by [11C]carboxylation of the corresponding enolate anion generated in situ from isopropenylacetate and MeLi, and purified by ion-exchange column resins.1-[11C]acetoacetate was synthesized with high radiochemical purity (95%) and specific activity (~ 66.6GBq/µmol, n = 30) with 35% radiochemical yield, decay corrected to end of synthesis. The total synthesis required ~ 16min. PET imaging studies were conducted with 1-[11C]acetoacetate in vervet monkeys to validate the radiochemical synthesis. Tissue uptake distribution was similar to that reported in humans.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2017 Tipo de documento: Article