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Biophysical and Computational Studies of the vCCI:vMIP-II Complex.
Nguyen, Anna F; Kuo, Nai-Wei; Showalter, Laura J; Ramos, Ricardo; Dupureur, Cynthia M; Colvin, Michael E; LiWang, Patricia J.
Afiliação
  • Nguyen AF; Departments of Molecular Cell Biology and Chemistry and Chemical Biology, and the Health Sciences Research Institute, University of California Merced 5200 North Lake Rd, Merced, CA 953402, USA. aankirskaia@ucmerced.edu.
  • Kuo NW; Departments of Molecular Cell Biology and Chemistry and Chemical Biology, and the Health Sciences Research Institute, University of California Merced 5200 North Lake Rd, Merced, CA 953402, USA. nicolekuo2010@gmail.com.
  • Showalter LJ; Departments of Molecular Cell Biology and Chemistry and Chemical Biology, and the Health Sciences Research Institute, University of California Merced 5200 North Lake Rd, Merced, CA 953402, USA. lshowalter@ucmerced.edu.
  • Ramos R; Departments of Molecular Cell Biology and Chemistry and Chemical Biology, and the Health Sciences Research Institute, University of California Merced 5200 North Lake Rd, Merced, CA 953402, USA. rramos4@ucmerced.edu.
  • Dupureur CM; Department of Chemistry & Biochemistry, University of Missouri-St. Louis, St. Louis, MO 63121, USA. dupureurc@umsl.edu.
  • Colvin ME; Departments of Molecular Cell Biology and Chemistry and Chemical Biology, and the Health Sciences Research Institute, University of California Merced 5200 North Lake Rd, Merced, CA 953402, USA. mcolvin@ucmerced.edu.
  • LiWang PJ; Departments of Molecular Cell Biology and Chemistry and Chemical Biology, and the Health Sciences Research Institute, University of California Merced 5200 North Lake Rd, Merced, CA 953402, USA. pliwang@ucmerced.edu.
Int J Mol Sci ; 18(8)2017 Aug 16.
Article em En | MEDLINE | ID: mdl-28813018
ABSTRACT
Certain viruses have the ability to subvert the mammalian immune response, including interference in the chemokine system. Poxviruses produce the chemokine binding protein vCCI (viral CC chemokine inhibitor; also called 35K), which tightly binds to CC chemokines. To facilitate the study of vCCI, we first provide a protocol to produce folded vCCI from Escherichia coli (E. coli.) It is shown here that vCCI binds with unusually high affinity to viral Macrophage Inflammatory Protein-II (vMIP-II), a chemokine analog produced by the virus, human herpesvirus 8 (HHV-8). Fluorescence anisotropy was used to investigate the vCCIvMIP-II complex and shows that vCCI binds to vMIP-II with a higher affinity than most other chemokines, having a Kd of 0.06 ± 0.006 nM. Nuclear magnetic resonance (NMR) chemical shift perturbation experiments indicate that key amino acids used for binding in the complex are similar to those found in previous work. Molecular dynamics were then used to compare the vCCIvMIP-II complex with the known vCCIMacrophage Inflammatory Protein-1ß/CC-Chemokine Ligand 4 (MIP-1ß/CCL4) complex. The simulations show key interactions, such as those between E143 and D75 in vCCI/35K and R18 in vMIP-II. Further, in a comparison of 1 µs molecular dynamics (MD) trajectories, vMIP-II shows more overall surface binding to vCCI than does the chemokine MIP-1ß. vMIP-II maintains unique contacts at its N-terminus to vCCI that are not made by MIP-1ß, and vMIP-II also makes more contacts with the vCCI flexible acidic loop (located between the second and third beta strands) than does MIP-1ß. These studies provide evidence for the basis of the tight vCCIvMIP-II interaction while elucidating the vCCIMIP-1ß interaction, and allow insight into the structure of proteins that are capable of broadly subverting the mammalian immune system.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vaccinia virus / Proteínas Virais / Herpesvirus Humano 8 / Complexos Multiproteicos / Quimiocina CXCL2 / Simulação de Dinâmica Molecular / Polarização de Fluorescência Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vaccinia virus / Proteínas Virais / Herpesvirus Humano 8 / Complexos Multiproteicos / Quimiocina CXCL2 / Simulação de Dinâmica Molecular / Polarização de Fluorescência Idioma: En Ano de publicação: 2017 Tipo de documento: Article