Optimization of Surfactant-Mediated, Ultrasonic-assisted Extraction of Antioxidant Polyphenols from Rattan Tea (Ampelopsis grossedentata) Using Response Surface Methodology.
Pharmacogn Mag
; 13(51): 446-453, 2017.
Article
em En
| MEDLINE
| ID: mdl-28839370
ABSTRACT
BACKGROUND:
Rattan tea is a medicinal plant that has been used for many years for the treatment of inflammation, fatty liver, tumor, diabetes, and hyperlipidemia.OBJECTIVE:
A green and novel approach based on surfactant-mediated, ultrasonic-assisted extraction (SM-UAE) was developed for the extraction of antioxidant polyphenols from Rattan tea. A nonionic surfactant Tween-80 was selected as extraction solvent. The antioxidant activity was measured by total phenolic content (TPC) and ferric-reducing/antioxidant capacity (FRAC) assay. MATERIALS ANDMETHODS:
Optimization of extraction parameters including concentration of solvent, ultrasonic time, and temperature were investigated by response surface methodology. The antioxidant activity was measured by TPC and FRAC assay.RESULTS:
The optimal extraction conditions were determined as 6.8% (v/v) of aqueous Tween-80, ultrasonic temperature of 54°C, and ultrasonic time of 28.8 min. Under these conditions, the highest TPC value of 360.4 mg gallic acid equivalent per gram of dry weight material (GAE/g DW) was recorded. Moreover, 6.8% (v/v) of aqueous Tween-80, ultrasonic temperature of 54.5°C, and ultrasonic time of 28.4 min were determined for the highest FRAC value of 478.2 µmol of Fe2+/g of weight material (µmol Fe2+/g DW). Compared with other methods, the TPC and FRAC values of 313.5 mg GAE/g DW and 389.6 µmol Fe2+/g DW were obtained by heat reflux extraction using ethanol as solvent, respectively, and 343.2 mg GAE/g DW and 450.1 µmol Fe2+/g DW were obtained by UAE using ethanol as solvent, respectively.CONCLUSION:
The application of SM-UAE markedly decreased extraction time or extraction cost and improved the extraction efficiency, compared with the other methods.SUMMARY:
Surfactant-mediated ultrasonic-assisted extraction of antioxidant polyphenols from Rattan TeaResponse surface methodology used to optimize parameters and study combined effectsOptimized surfactant-mediated ultrasonic-assisted extraction process enhances the antioxidant phenolics extraction in less time. Abbreviations used SM-UAE Surfactant-mediated ultrasonic-assisted extraction; TPC total phenolic content; FRAC Ferric reducing antioxidant capacity; RSM Response surface methodology; BBD Box-Behnken design.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article