[Application of rapid PCR to authenticate Ranae Oviductus].
Zhongguo Zhong Yao Za Zhi
; 42(13): 2467-2472, 2017 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-28840685
ABSTRACT
Rapid allele-specific PCR primer was designed base on Cytb 155 A/T single nucleotide polymorphism, DNA was extracted by alkaline lysis and the PCR reaction systems including denatured and annealing temperature and cycle numbers were optimized. The results were performed to authenticate Ranae Oviductus and its 4 adulterants. When 100×SYBR Green I was added in the PCR product at 90 â denatured 3 s, 62 â annealing 20 s and 32 cycle. Ranae Oviductus visualized strong green fluorescence under 365 nm UV lamp whereas adulterants appeared negative. The whole process can be completed in 40 minutesï¼The established method provides the technical support for authentication of the Ranae Oviductus.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oviductos
/
Ranidae
/
Reação em Cadeia da Polimerase
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2017
Tipo de documento:
Article