Targeted Lipidomic Analysis of Myoblasts by GC-MS and LC-MS/MS.
Methods Mol Biol
; 1668: 39-60, 2017.
Article
em En
| MEDLINE
| ID: mdl-28842901
ABSTRACT
Lipids represent â¼10% of the cell dry mass and play essential roles in membrane composition and physical properties, energy storage, and signaling pathways. In the developing or the regenerating skeletal muscle, modifications in the content or the flipping between leaflets of membrane lipid components can modulate the fusion capacity of myoblasts, thus constituting one of the regulatory mechanisms underlying myofiber growth. Recently, few genes controlling these qualitative and quantitative modifications have started to be unraveled. The precise functional characterization of these genes requires both qualitative and quantitative evaluations of a global lipid profile. Here, we describe a lipidomic protocol using mass spectrometry, allowing assessing the content of fatty acids, glycerophospholipids, and cholesterol in the routinely used C2C12 mouse myoblast cell line, or in primary cultures of mouse myoblasts.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Colesterol
/
Glicerofosfolipídeos
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Mioblastos
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Ácidos Graxos
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Lipídeos de Membrana
Tipo de estudo:
Qualitative_research
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article