Development of high-throughput and high sensitivity capillary gel electrophoresis platform method for Western, Eastern, and Venezuelan equine encephalitis (WEVEE) virus like particles (VLPs) purity determination and characterization.
Electrophoresis
; 38(20): 2610-2621, 2017 10.
Article
em En
| MEDLINE
| ID: mdl-28842921
ABSTRACT
In this paper, we describe development of a high-throughput, highly sensitive method based on Lab Chip CGE-SDS platform for purity determination and characterization of virus-like particle (VLP) vaccines. A capillary gel electrophoresis approach requiring about 41 s per sample for analysis and demonstrating sensitivity to protein initial concentrations as low as 20 µg/mL, this method has been used previously to evaluate monoclonal antibodies, but this application for lot release assay of VLPs using this platform is unique. The method was qualified and shown to be accurate for the quantitation of VLP purity. Assay repeatability was confirmed to be less than 2% relative standard deviation of the mean (% RSD) with interday precision less than 2% RSD. The assay can evaluate purified VLPs in a concentration range of 20-249 µg/mL for VEE and 20-250 µg/mL for EEE and WEE VLPs.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Eletroforese Capilar
/
Vírus da Encefalite Equina do Leste
/
Vírus da Encefalite Equina Venezuelana
/
Vírus da Encefalite Equina do Oeste
/
Ensaios de Triagem em Larga Escala
/
Vacinas de Partículas Semelhantes a Vírus
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
País/Região como assunto:
America do sul
/
Venezuela
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article