Redirecting Killer Tâ
Cells through Incorporation of Azido Sugars for Tethering Ligands.
Chembiochem
; 18(21): 2082-2086, 2017 11 02.
Article
em En
| MEDLINE
| ID: mdl-28862366
ABSTRACT
The genetic expression of chimeric antigen receptors (CARs) on the surfaces of Tâ
cells enables the redirection of Tâ
cell specificity. To enhance the versatility of Tâ
cells as tumor-specific killers, we developed a nongenetic approach by which azide-containing sialic acids were metabolically incorporated into Tâ
cells to modify cellular sialyl glycans. After successful display of these moieties on the Tâ
cells, small-molecule ligands such as RGD and folate (as proof-of-concept, rather than supersized antibodies) were clicked orthogonally, leading to highly selective time- and dose-dependent cytotoxicity to integrin αv ß3 - and folate-receptor-positive cells, respectively. This chemical approach provides a facile platform for rational design of tumor-specific cytotoxic Tâ
cells for targeted immunotherapy.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligopeptídeos
/
Polissacarídeos
/
Ácidos Siálicos
/
Azidas
/
Linfócitos T Citotóxicos
/
Ácido Fólico
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article