Investigation of the mechanism of the SpnF-catalyzed [4+2]-cycloaddition reaction in the biosynthesis of spinosyn A.
Proc Natl Acad Sci U S A
; 114(39): 10408-10413, 2017 09 26.
Article
em En
| MEDLINE
| ID: mdl-28874588
ABSTRACT
The Diels-Alder reaction is one of the most common methods to chemically synthesize a six-membered carbocycle. While it has long been speculated that the cyclohexene moiety found in many secondary metabolites is also introduced via similar chemistry, the enzyme SpnF involved in the biosynthesis of the insecticide spinosyn A in Saccharopolyspora spinosa is the first enzyme for which catalysis of an intramolecular [Formula see text]-cycloaddition has been experimentally verified as its only known function. Since its discovery, a number of additional standalone [Formula see text]-cyclases have been reported as potential Diels-Alderases; however, whether their catalytic cycles involve a concerted or stepwise cyclization mechanism has not been addressed experimentally. Here, we report direct experimental interrogation of the reaction coordinate for the [Formula see text]-carbocyclase SpnF via the measurement of [Formula see text]-secondary deuterium kinetic isotope effects (KIEs) at all sites of [Formula see text] rehybridization for both the nonenzymatic and enzyme-catalyzed cyclization of the SpnF substrate. The measured KIEs for the nonenzymatic reaction are consistent with previous computational results implicating an intermediary state between formation of the first and second carbon-carbon bonds. The KIEs measured for the enzymatic reaction suggest a similar mechanism of cyclization within the enzyme active site; however, there is evidence that conformational restriction of the substrate may play a role in catalysis.
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01-internacional
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MEDLINE
Assunto principal:
Macrolídeos
/
Reação de Cicloadição
/
Metiltransferases
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article