Novel amine-functionalized iron trimesates with enhanced peroxidase-like activity and their applications for the fluorescent assay of choline and acetylcholine.
Biosens Bioelectron
; 100: 161-168, 2018 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-28888178
ABSTRACT
We herein describe novel amine-grafted metal-organic frameworks (MOFs) as a promising alternative to natural peroxidase enzyme and their applications for a fluorescent assay of choline (Cho) and acetylcholine (ACh). Among diverse amine-functionalized MOFs, N,N,N',N'-tetramethyl-1,4-butanediamine (TMBDA)-functionalized MIL-100(Fe) (TMBDA-MIL-100(Fe)) exhibited the highest peroxidase activity by developing intense fluorescence from Amplex UltraRed (AUR) in the presence of H2O2, which was presumably due to the synergetic effect of the enhanced negative potential and precisely controlled molecular size of the grafted diamine. Based on the excellent peroxidase-like activity of TMBDA-MIL-100(Fe), choline and ACh were reliably determined down to 0.027 and 0.036µM, respectively. Furthermore, practical applicability of this strategy was successfully demonstrated by detecting choline and ACh in spiked samples of milk and serum, respectively. This work highlights the advantages of amine-grafted MOFs for the preparation of biomimetic catalysts, extending their scope to biosensor applications.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Acetilcolina
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Putrescina
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Colina
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Compostos de Ferro
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Peroxidase
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article