Lactobacillus curtus sp. nov., isolated from beer in Finland.
Int J Syst Evol Microbiol
; 67(10): 3899-3906, 2017 Oct.
Article
em En
| MEDLINE
| ID: mdl-28895518
ABSTRACT
A Gram-stain-positive, catalase-negative and short-rod-shaped organism, designated VTT E-94560, was isolated from beer in Finland and deposited in the VTT culture collection as a strain of Lactobacillus rossiae. However, the results of 16S rRNA gene sequence analysis showed that VTT E-94560 was only related to Lactobacillus rossiae JCM 16176T with 97.0â% sequence similarity, lower than the 98.7â% regarded as the boundary for the species differentiation. Additional phylogenetic studies on the pheS gene, rpoA gene and 16S-23S rRNA internally transcribed spacer region further reinforced the taxonomically independent status of VTT E-94560 and its related Lactobacillus species including L. rossiae and Lactobacillus siliginis. Strain VTT E-94560 also exhibited several differences in its carbohydrate fermentation profiles from those related Lactobacillus species. In addition, DNA-DNA relatedness between VTT E-94560 and these two type strains was 4â% (L. rossiae JCM 16176T) and 12â% (L. siliginins JCM 16155T), respectively, which were lower than the 70â% cut-off for general species delineation, indicating that these three strains are not taxonomically identical at the species level. These studies revealed that VTT E-94560 represents a novel species, for which the name Lactobacillus curtus sp. nov. is proposed. The type strain is VTT E-94560T (=JCM 31185T).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
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Cerveja
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Microbiologia de Alimentos
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Lactobacillus
País/Região como assunto:
Europa
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article